补气活血方药对衰老急性肺损伤大鼠模型血液及肺组织中ET-1和NO含量的影响

Effect of invigorating Qi and promoting blood circulation on ET-1 and NO contents in the blood and lung tissue of aged rats with acute lung injury

目的探讨补气活血方药对衰老急性肺损伤(ALI)的作用机制。方法采用腹腔注射D-半乳糖(D-gal)复制衰老大鼠模型,随机分为衰老组、脂多糖(LPS)组、中药高剂量组、中药低剂量组、氨基胍(AG)组,并设不加处理的正常对照组。以高、低剂量补气活血方药和AG进行干预后,尾静脉注射LPS致大鼠ALI。测定血液及肺组织中ET-1和NO含量。结果衰老组与正常对照组、LPS组与衰老组比较,血液及肺组织中ET-1和NO的含量均有显著性差异(P<0.01)。用药干预后,治疗各组血浆中ET-1的含量与LPS组相比,均有统计学意义(P<0.05或P<0.01),AG组血清及肺组织中NO的含量较LPS组明显降低(P<0.01)。结论衰老性ALI的发生与体内ET-1和NO含量变化有关,补气活血方药可通过减少ET-1的合成与释放,调节ET和NO的平衡,从而促进损伤修复。

Objective To observe ET-1 and NO contents in the blood and lung tissue of aged rats with acute lung injury （ALI）, to make research on the functional mechanism of treating experimental aged ALI with Chinese medicine for invigorating qi and promoting blood circulation. Methods Animal model of aged rats, duplicated by D-gal injection, were randomly divided into model, LPS, high dose, low dose, AG group, and normal control group without any treatment. After intervened by high and low dose of Chinese medicine for invigorating qi and promoting blood circulation as well as AG, and ALI were made by injecting LPS. ET-1 ＆ NO contents were determined in blood and lung tissue of each group. Results The ET-1 ＆ NO contents in the blood and lung tissue of model group were significantly higher than those of nomad group （P 〈 0. 01 ） ; After intervention, ET-1 contents in the blood plasma of treatment groups were significantly reduced （ P 〈 0. 05 or P 〈 0. 01 ） and NO contents in blood serum and lung tissue of AG group were significantly reduced （ P 〈 0. 01 ） compared with those in LPS group. Conclusions The occurrence of aged rats with ALI is relative with the change of ET-1 ＆ NO. The prescription of invigorating qi and promoting blood circulation can reduce synthesis and release of ET-1 and NO, balance the ET-1 and NO, and improve the condition of the blood circulation.