摘要
从糙皮侧耳Pleurotus ostreatus Pm039菌丝体中分离纯化到一种疏水蛋白并命名为Po.HYD1,SDS-PAGE显示其分子量约15kDa。Po.HYD1具有高度的表面活性,100μg/mL浓度下能够降低水表面张力至25.5mN/m。在1~100μg/mL浓度范围内存在6μg/mL和24μg/mL两个关键浓度,说明了不同浓度范围内自组装条件的改变。水接触角测定证明了Po.HYD1自组装膜的包被能够逆转固体表面的可湿润性。原子力显微镜分析揭示了Po.HYD1在云母表面形成厚度4.2±0.1nm＂小杆层＂;在高定向热裂解石墨表面形成厚度3.2～3.8nm吸附层;在剧烈振荡诱导下的水溶液中形成形状相似、取向一致但体积大小不等的＂耳型＂颗粒。
A hydrophobin designated as Po.HYD1 was purified from Pleurotus ostreatus Pm039. SDS-PAGE analysis showed an approximate molecular mass of 15kDa. Po.HYD1 showed highly surface activity, and it was able to decrease water surface tension to 25.5mN/m at concentration of 100μg/mL. Two crucial concentrations, 6μtg/mL and 241μg/mL, were found by protracting the concentration-surface tension curve in range of 1-100μg/mL, which maybe indicate different conditions concerning interfacial self-assembly. Measurement of water contact angles suggested Po.HYD1 can reverse surface wettability through coating solid materials. Atomic force microscopy revealed Po.HYD1 formed distinct assemblages at different hydrophilic-hydrophobic interfaces. It formed ＂rodlet layer＂ with thickness of 4.2±0. lnm on mica surface, formed adsorption layer with thickness of 4.2±0.1nm on highly oriented pyrolytic graphite surface, and formed ear-like particles with similar shape, identical orientation and various sizes in aqueous solution by induction of vigorous shaking.
出处
《菌物学报》
CAS
CSCD
北大核心
2007年第4期557-564,共8页
Mycosystema
基金
国家自然科学基金资助项目(No.39770530
No.30170658)
关键词
糙皮侧耳
疏水蛋白
平衡表面张力
水接触角
原子力显微镜
Pleurotus ostreatus
hydrophobin
equilibrium surface tension
water contact angle
atomic force
