川芎嗪对凝血酶诱导的血管平滑肌细胞增殖的影响

Effect of Tetramethylpyrazine on vascular smooth muscle cell proliferation induced by thrombin

目的观察川芎嗪(Tetramethylpyrazine,TMP)对凝血酶诱导的血管平滑肌细胞(vascular smooth muscle cell,VSMC)增殖的影响及可能机制。方法酶消化法培养兔胸主动脉VSMC。WST-8比色法、流式细胞仪及免疫细胞化学测定TMP对凝血酶诱导的VSMC增殖、细胞周期及c-fos表达的影响。结果TMP浓度依赖性地抑制凝血酶诱导的VSMC增殖,20mg/L TMP能够抑制凝血酶诱导的VSMC G0/G1期向S期的转变及c-fos表达。结论TMP浓度依赖性地抑制凝血酶诱导的VSMC增殖、机制与其抑制c-fos表达和抑制VSMC细胞周期G0/G1期向S期的转变有关。

AIM To investigate the effect of tetramethylpyrazine （TMP） on the proliferation of vascular smooth muscle cell（VSMC） induced by thrombin and the possible underlying mechanism. METHODS VSMC were isolated from rabbit aorta using enzyme digestion method. WST-8 colorimetry was carried out to evaluate the cell growing state. Cell cycle analysis was evaluated by flow cytometry and immunocytochemical staining was used to study the expression of c-fos. RESULTS TMP inhibited the proliferation of VSMC induced by thrombin in a dose-dependent manner. 20 mg/L TMP could delay the transition from G0/G1 phase into S phase and decrease the expression of c-fos induced by thrombin. CONCLUSION TMP inhibit the proliferation of VSMC induced by thrombin in a dose-dependent manner, at least in part by inhibiting the expression of c-fos induced by thrombin and preventing the transition from G0/G1 into S phase of VSMC.