抗哇巴因鸡蛋黄IgY与兔抗体IgG在酶联免疫检测中的比较

COMPARISION BETWEEN ANTI-OUABAIN EGG YOLK(IgY) AND RABBIT ANTIBODY(IgG) IN ENZYME-LINKED IMMUNOSORBENT ASSAY

目的:探讨提高内源性哇巴因(EO)检测方法的准确性及特异性,为哇巴因的研究打下基础。方法:分别制备出鸡蛋黄抗哇巴因多克隆抗体及兔抗体。然后采用酶联免疫吸附法(ELISA)比较两种抗体检测内源性哇巴因的准确性及特异性。结果:采用IgY检测EO的平均批内变异系数为2.03%,批间变异系数为2.34%。兔抗体IgG则分别为2.83%、3.29%;两者均与氢化可的松及地塞米松无交叉结合反应,与西地兰和地高辛分别存在3.45%vs5.95%、3.20%vs5.20%的交叉反应。结论:IgY比兔抗体ELISA检测内源性哇巴因的特异性及准确性较高。

To improve specificity and accuracy of endogenous ouabain measurement assay. Methods： Anti-ouabain polyclonal antibody egg yolk（IgY） and anti-ouabain rabbit antibody（IgG） were prepared respectively. In the presence of two kinds of antibody, then the specificity and accuracy of enzyme-linked immunosorbent assay（ELISA） were compared. Results： The ELISA, in the presence of IgY, provided a sensitivity of the average intraassay coefficient of variation（CV） was 2.03 %, and the inter-assay CV was 2.34 % respectively. In contrast, IgG were 2.83 % and 3.29 %. No significant interferences were observed with hydrocortisone and dexamethasone. There was 3.45% vs 5.95% ,3.20% vs 5.20% of crossreaction with cedilanid and digoxin. Conclusion： The specificity and accuracy of ELISA, in which IgY was used, were more better than IgG.