摘要
研究了培养基、植物生长调节素以及接种量对Vitex glabrata R.Br.悬浮培养细胞的生长情况以及对20-羟基蜕皮激素形成的作用。当细胞在添加有2.0mg/LBAP(6-苯甲酸嘌呤)和1.0mg/L2,4-D的Gamborg’s B5培养基中培养时细胞生长和20-羟基蜕皮激素的形成达到了最高水平。当接种量为20%PCV(积压细胞体积)时观察到了20-羟基蜕皮激素的最高产量,大约是1.1mg/(L.d)。实验数据也表明当接种量增加到20%PCV时,产量提高了7倍。
The effects of the cultivation media, plant growth regulators and inoculum size on the cell growth and 20- hydroxyecdysone production in suspension cultures of Vitex glabrata R. Br. were investigated. The cell growth and 20- hydroxyecdysone formation reach the highest when cells are cultured in the Gamborg＇s B5 medium supplemented with 2.0mg/L BAP （6-benzylaminopurine） and 1.0mg/L 2,4-D. The maximum 20-hydroxyecdysone productivity, of about 1. 1mg/L/day, was observed in the culture with 20% PCV （packed cell volume） of inoculum size. These data also show that the increment of the inoculum size to 20% PCV could increase the productivity in 7-folds.
出处
《生物工程学报》
CAS
CSCD
北大核心
2007年第6期1033-1036,共4页
Chinese Journal of Biotechnology
