麦冬提取物的抗氧化活性和抑菌作用研究

Antioxidant Activities of Extracts from Ophiopogon japonicus

采用乙醇回流提取,热水煮提和稀碱提取方法分别从麦冬中提取出醇溶性提取物,水溶性提取物和稀碱提取物。利用超氧阴离子清除实验和羟自由基清除实验研究3种提取物的抗氧化活性。结果表明,稀碱提取物和热水提取物均有较强的羟自由基清除能力,且高于V_c的清除能力。稀碱提取物在低浓度时与V_c相比有更强的超氧阴离子清除作用。乙醇提取物对革兰氏阳性菌枯草芽孢杆菌有较强的抑制作用,最低抑菌浓度是1 mg/mL。而热水提取物对革兰氏阴性菌大肠杆菌和真菌假丝酵母有一定的抑制作用,最低抑菌浓度分别是9.7mg/mL与6.5mg/mL。

The ethanol soluble extracts, water soluble extracts and alkali extracts were obtained from the Ophiopogonjaponicus by ethanol-circumfluence extraction, boiling-water extraction and alkali extraction. On the basis of superoxide radical assay and hydroxyl radical assay, their antioxidant activities were investi- gated. Among them, alkali extracts and water soluble extracts had higher inhibitory activities than that of Vc for hydroxyl radical. In addition, alkali extracts has higher inhibitory activity of superoxide radical than Vc at low concentration. The ethanol-soluble extracts had remarkable antimicrobial activities for Bacillus subtilis Cohn, and the MIC was lmg/mL. E. coli and Candida glycerolgenesis can be strongly inhibited by water soluble extracts, at the MIC 9.7mg/mL and 6.5mg/mL respectively.