稀酸水解玉米皮制备丁二酸发酵糖液的研究

Preparation of Saccharifying Liquid for Succinic Acid by Fermentation from Cornhusks Sulphuric Acid Hydrolysis

研究了稀酸水解玉米皮制备丁二酸发酵糖液的工艺条件,通过正交试验及优化调整得到稀酸水解玉米皮的优化工艺条件:水解温度110℃、加酸量1%、粒径20～40目、反应时间90min,总糖收率90.37%,总糖浓度85g/L。其糖液经活性炭脱色,脱色率达92.27%,脱色的总糖损失率低于5%,糠醛含量仅为0.236g/L。经厌氧发酵实验初步验证,玉米皮水解液可替代葡萄糖作为丁二酸发酵的碳源。

The techniques of saccharifying liquid preparation from cornhusks by acid hydrolysis were studied. The optimum parameters for the hydrolysis based on the yield of total sugar were determined by means of orthogonal experiments and regulative experiments. The best conditions of hydrolysis were as follows： hydrolysis temperature 110℃, vitriol concentration 1. 0%, particle diameter 20 - 40 meshes, reaction time 90min. The yield of total sugar was 90.37% and the concentration of total sugar was 85 g/L. The saccharifying liquid from cornhusks was decolorized by active carbon. Yield of decolorization was 92.27%. Losing rate of total sugar was below 5% and the concentration of furfurol was 0. 236 g/L. The shaking flasks experiment for the production of succinic acid was carried out and the results showed that the saccharifying liquid could be used as the carbon source substitute for microbe fermentation of succinic acid.