糖脂产生菌的筛选及发酵液的表面活性研究

Screening a Strain Producing Giycolipids and Studying the Surface Activity of Culture Broth

从来源不同的土样和污水中,分离得到1株酵母菌Y_(2A)。摇瓶发酵培养,发现该菌发酵液具有很好的排油活性,并且能将发酵液的表面张力从72mN/m降为37.4mN/m。薄层层析、紫外光谱、红外光谱分析以及菌种鉴定,表明该菌是1株未见报道的糖脂产生菌拟成克酵母(Wickerhamiella domercqiae)Y_(2A)。该糖脂在pH 2～8,12%盐浓度和120℃下仍然保持良好的表面活性,并且对原油有较好的乳化能力。

A yeast strain Y2A was isolated from samples from soil and wastewater. After fermentation, the culture broth of Y2A had good oil film-collapsing activity and the surface tension was decreased from 72mN/m to 37.4mN/m. Furthermore, the strain was identified as glycolipids-producing Wickerhamiella domercqiae Y2A by TLC, UV and infrared spectra analysis. Our data also demonstrated that the NaCl concentration （〈12%）, pH （2-8） and high temperature （-120℃） did not affect the surface tension of Y2A culture broth. And the culture broth can emulsify the crude oil.