高梁籽粒提取物抗氧化活性的研究

Antioxidant Properties of Sorghum Groats Extract

以水和甲醇为溶剂提取高粱籽粒中的多酚类化合物,测定了提取物中的总酚含量、还原力、Fe^(2+)络合能力,以及提取物对1,1-苯基-2-苦肼基自由基(DPPH)、2,2-联氮-3-乙苯-二噻唑-6磺酸(ABTS)、定位和非定位羟基自由基的清除能力。结果表明:高梁籽粒提取物中总酚含量范围为3.87%～38.87%;高粱籽粒甲醇提取物对DPPH和ABTS自由基的清除能力以及还原力均高于水提取物,但水提取物的络合力高于甲醇提取物。高粱籽粒提取物对定位和非定位羟基自由基介导的2-脱氧核糖均有保护作用,其保护机理呈多样性。

Polyphenol compounds were extracted from the sorghum groats by water and methanol . Total phenolic content ,reducing power ,chelating activity, 1,1-diphenyl-2-pierylhydrazyl （DPPH） radical scavenging activity, 2,2-azino-bis-（3-ethylbenzothiazoline-6-sulphonic acid） （ABTS） radical cation scavenging activity and inhibition of site-specific and non-site-specific hydroxyl radical-mediated 2-deoxy-D-ribose degradation of the extract from the sorghum groats have been determined. Total phenolic content in the extract were from 3.87 to 38.87%. DPPH radical scavenging activity, ABTS radical cation scavenging activity and reducing power of methanol extract were higher than water extract, while water extract exhibited higher chelating activity. It was founded that sorghum groats extract could inhibited the site-specific and non-site-specific hydroxyl radical-mediated 2-deoxy-D-ribose degradation, however its mechanism was complicated.