大豆异黄酮与Vc对糖尿病大鼠抗氧化能力影响的比较

Effects comparison of antioxidative ability between soybean isoflavone and Vc in diabetic rats

比较大豆异黄酮(soybean isoflavone,SIF)与Vc对糖尿病大鼠抗氧化能力的影响。方法:用四氧嘧啶(ALX)建立糖尿病动物模型,正常对照组(N)和糖尿病对照组(DC)以蒸馏水灌胃,大豆异黄酮组(SIF)和Vc组(VC)分别以100mg/(kg·d)的SIF溶液和10.4mg/(kg·d)的Vc溶液灌胃。实验6周后,分别测血清及肝脏中总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和黄嘌呤氧化酶(XOD)活性以及丙二醛(MDA)含量。结果:DC组灌胃后血糖较灌胃前明显升高(P<0.05),SIF组和Vc组血糖均明显低于灌胃前和DC组(P<0.05)。与DC组相比,SIF组血清和肝脏GSH-Px和SOD活性及肝脏T-AOC明显增强(P<0.05),血清及肝脏MDA含量均明显降低(P<0.05)。Vc组血清GSH-Px和肝脏SOD活性、T-AOC明显高于DC组(P<0.05),MDA含量明显低于DC组(P<0.05)。SIF组和VC组在血清及肝脏T-AOC、SOD、GSH-Px、XOD活性和MDA含量上无明显差异(P>0.05)。结论:大豆异黄酮具有降血糖和增强机体抗氧化能力,其抗氧化效果与Vc相当。大豆异黄酮通过降低血糖、改善抗氧化酶活性和抑制脂质过氧化等作用发挥对糖尿病治疗作用。

Objective： To observe the effects of soybean isoflavone（SIF） on antioxidative ability in diabetic rats induced by Alloxane. Methods： DM model was built by i.p ALX. Distilled water was given to normal control group（N） and diabetic control group（DC） by gavage; 100mg/（kg·d） islflavone and 10.4mg/（kg·d） Vc were supplemented to SIF group and Vc group separately by means of gavage. After 6 weeks, T-AOC, SOD, GSH-Px, XOD and MDA of serum and liver in every group were tested. Results： Blood glucose level in SIF group and Vc group were significantly lower than those in DC group（P〈0.05）. The level of blood glucose in DC group was remarkably higher than before（P〈0.05）. Comparing with DC group, GSH-Px and SOD activities of serum and liver,T-AOC level of liver in SIF group were higher remarkably（P〈0.05）; GSH-Px and SOD activities, T-AOC were remarkably higher than DC group（P〈0.05）; MDA level of serum and liver in SIF group and Vc group were much lower than that in DC group（P〈0.05）. SIF group and Vc group had no significant difference in serum and liver T-AOC, SOD, GSH-Px, XOD activities and MDA content（P〉0.05）. Conclusion： SIF has the ability of decreasing blood glucose level and improving antioxidant ability, its effect is as well as Vc. SIF can therapy DM through lowering blood glucose ,recovering of antioxidant enzymes abilities and restraining lipid oxidation.