冲击伤复合缺氧后大鼠血清TNF-α与IL-8含量的变化

Changes of TNF-α and IL-8 in blood plasma after blast injury combined with hypoxia in rats

目的探讨大鼠冲击伤、冲击伤复合缺氧后血清TNF-α、IL-8的含量变化特点及其对伤情的判断意义。方法80只大鼠按随机数字表法分为3组,即正常对照组(NG,n=8)、单纯冲击伤组(BG,n=36)和冲击伤复合缺氧组(BHG,n=36)。致伤组动物用BST-Ⅰ型生物激波管致冲击伤,BHG组大鼠再置入体积分数为12.5%的低氧舱,于伤后1、3、6h处死大鼠,采用大体、光镜和电镜方法观察伤后肺病理学变化和肺含水率,并用ELISA法监测血清TNF-α和IL-8含量变化。结果受伤动物可见明显的肺出血、肺水肿,BG和BHG组动物肺含水率及血清TNF-α和IL-8含量均明显高于NG组(P<0.01),其中BHG组血清TNF-α和IL-8含量在伤后6h又显著高于BG组(P<0.05)。结论冲击伤复合缺氧能明显增加血清TNF-α和IL-8含量,其水平变化可能与肺损伤程度密切相关。

Objective To explore the changes and diagnostic value of tumor necrosis factor （TNF）-α and interleukin （IL）-8 levels in rat plasma after blast injury or blast injury combined with hypoxia. Methods Eighty Wistar rats were randomly divided into 3 groups ： the normal control group （ NG, n = 8）, the blast injury group （BG, n = 36）, and the blast-hypoxia injury group （BHG, n = 36）. The rats were inflicted with blast injury by BST-Ⅰ bio-shock tube alone in BG, or subsequently were immediately put into the hypoxic cabin （volume fraction of O2was 12.5% ） in BHG. The rats were killed at 1, 3, 6 h after injury, respectively. The pathological changes of the lung tissues and the water content in lung were observed with gross examination, light microscopy and electron microscopy. The changes of TNF-α and IL-8 levels in rat plasma were analyzed by ELISA. Results There were obvious hemorrhage and edema in the lung tissues of the injured rats. The water content in lung and the plasma levels of TNF-α and IL-8 were obviously increased in BG and BHG as compared to NG （P 〈0.01 ）, and the content of TNF-α and IL-8 in blood plasma was higher in BHG than that in BG at 6 h （ P 〈 0. 05）. Conclusion Blast injury combined with hypoxia can significantly increase the levels of TNF- α and IL-8 in rat plasma that are in consistent with the severity of lung injury.