抑制Kit信号通路对豚鼠膀胱间质细胞增殖及自主节律性运动的影响

Effects of inhibiting Kit signal pathway on proliferation and spontaneous rhythmic motility of interstitial cells in bladder of guinea pigs

目的探讨抑制Kit表达对豚鼠膀胱间质细胞(interstitial cells,ICs)增殖及自主节律性运动的影响。方法成年豚鼠分别予伊马替尼灌胃20d和40d,免疫荧光、Western blot和体外灌流观察间质细胞形态和功能的变化。结果正常豚鼠膀胱壁内可见较多的间质细胞,用药20d和40d后,细胞数量逐渐减少,Kit蛋白的表达逐渐降低,同时,膀胱的自主节律性运动受到抑制。结论膀胱内含间质细胞,这些细胞可能是膀胱运动的起搏细胞,其细胞表面表达的Kit蛋白对间质细胞的存活和功能维持都具有重要的作用。

Objective To investigate the role of Kit signal pathway in the proliferation and spontaneous rhythmic motility of interstitial cells （ICs） in the bladder of guinea pigs. Methods Fourteen Guinea pigs aged 45 - 60 days were administered with Imatinib intragastrically for 20 and 40 days. Three were fed on normal sa- line as control. The morph and number of ICs were observed by immunofluorescence, Kit protein expression was detected by Western blot and spontaneous rhythmic motility of bladder were investigated in in vitro organ bath. Results Quite a few ICs were observed in the bladder of controls. After administration of Imatinib, the amount of ICs and the expression of Kit protein decreased gradually, and the spontaneous rhythmic contraction weakened. Conclusion ICs might be the pacemaker of bladder in guinea pigs. Kit protein might play an important role in the survival and functional maintenance of ICs.