短芒大麦DREB2基因的克隆及其转基因烟草的鉴定

Clone of HbDREB2 Gene of Hordeum brevisubulatum and Identification of Its Transgenic Tobaccos

以短芒大麦为材料,应用RT-PCR技术扩增获得792 bp的DREB2基因,构建了重组植物表达载体pBI-DREB2。通过农杆菌转化法将其导入烟草,并利用PCR、PCR-Southern和RT-PCR技术对获得的20株卡那霉素阳性转基因烟草进行分子鉴定。结果表明:短芒大麦DREB2基因已整合到烟草基因组中,并能在转录水平上表达。

We constructed a recombinant botany expression vector of DREB2 gene isolated from Hordeum brevisubulatum which was 792 bp, by RT - PCR technique and identified 20 of kan resistant positive transgenic tobaccos by molecular biology methode of PCR, PCR-Sourthem and RT- PCR. These results indicated that HbDREB2 gene has integrated into the tobacco genome and transcribed successfully