裸小鼠人侵袭性结肠癌模型的建立

The establishment of ultra-metastatic nude mice model of human colon cancer

目的:建立裸小鼠人结肠癌高侵袭模型,为侵袭性结肠癌的治疗研究提供可靠的模型动物。方法:将人类结肠癌细胞株HT29的细胞悬液接种于BALB/c小鼠脾包膜下和BALB/c nu/nu小鼠皮下,以前者肝转移肿瘤组织和后者皮下肿瘤组织作为移植瘤材,通过外科原位移植技术分别种植于实验组和对照组BALB/c nu/nu小鼠盲肠浆膜下层,比较两组小鼠在原位成瘤率、肝转移率、淋巴转移率、腹腔种植率及脾、肺转移率等方面是否存在显著性差异。结果:术后第14日,实验组原位成瘤率和肝脏转移率(100%、65%)高于对照组(75%、30.8%)(P<0.05);术后第21日,实验组原位成瘤率、肺脏转移率及脾脏转移率(100%、5%、15%)与对照组(85%、0、0)相比无显著性差异(P>0.05),但其肝脏转移率、淋巴转移率以及腹腔种植率(90%、65%、60%)均高于对照组(41.2%、23.5%、23.5%)(P<0.05)。结论:将免疫功能正常小鼠筛选出的结肠癌肝转移组织用于建立裸小鼠的原位移植癌模型,能够模拟高侵袭性结肠癌的生物学特性,并为其相关研究提供可靠的模型动物。

Objective： To establish an ultra - metastatic nude mice model of human colon cancer. Methods： Tumor cell line HT29 of human colon cancer, which had been made into 0.2ml cell suspension（3×10^6cells/ml）, was inoculated isubserously into the spleen in BLAB/c mice and subcutaneouly in BALB/c nu/nu mice to obtain liver metastatic and subcutaneous specimen. Then, fragments in 1 mm^3 of above specimen were implanted subserously in the colon in 20 BALB/c nu/nu mice of the experimental group and the control group respectively by surgical orthotopic implantation（SOI）. All the mice were executed after 21 days and their organs：colon, liver, lung, etc. were examined to define the difference of the growth and metastasis situation between the two groups. Results： On the 14th day postoperation, the tumor take rate and liver metastatic rate（ 100%, 65% ） in the experimental group were obviusly higher than those（75% ,30.8% ） in the control group; on the 21th day postoperation, the tumor take rate was no statistical difference between the two groups, but the metastatic rate to liver, lymph nodes and abdominal cavity （90% , 65%, 60% ） were obviusly higher than those（41.2% ,23.5% ,23.5% ） in the control group. Conclusion： The method of SOl in nude mice with the selective cell lines by cells orthotopic inoculation in immuno - normal mice can establish an ultra -metastatic animal model resembling the original human colon cancer histologically.