支气管肺泡灌洗液hTERT mRNA检测对肺癌诊断的意义

The diagnostic value of hTERT mRNA expression level in bronchoalveolar lavage fluid to lung cancer

目的:研究支气管肺泡灌洗液(brocho-alveolar larage fluid,BALF)人端粒酶催化亚单位(hTERTmRNA)表达水平对肺癌诊断的意义。方法:半定量RT-PCR检测31例肺癌、18例肺炎患者支气管肺泡灌洗液的hTERTmRNA表达水平。结果:肺癌组、肺炎组hTERT mRNA的水平分别为:0.92±0.12、0.54±0.15。肺癌组与肺炎组hTERTmRNA的表达量相比较有显著性差异(P<0.01)。对肺癌诊断的敏感度83.87%;特异度100%;阳性预测值100%,阴性预测值78.26%;准确度89.8%。结论:检测肺癌患者支气管肺泡灌洗液hTERTmRNA的表达水平是诊断肺癌的方法之一。

Objective.To investigate the diagnostic value of hTERT mRNA expression level of BALF （brocho - alveolar larage fluid） of lung cancer. Methods- RT -PCR was used to detect hTERT mRNA expression quantity in the BALF of lung cancer group and pneumonia group. Results： The expression level of hTERT mRNA of BALF in lung cancer group was 0.92 ±0.12 and pneumonia group was 0.5446 ±0. 1459. Significant difference was found between the two groups （ P 〈 0. 01 ）. Sensitity was 83.87% ; Specificity was 100% ; Positive predictive value was 100% ;Negative predictive value was 78.26% ;Accuracy was 89.8%. Conlusion： Detecting hTERT mRNA expression of BALF was one method for lung cancer diagnosis.