陕北胃癌高危人群胃粘膜组织中突变型p53蛋白的表达与Hp感染的关系

The relationship between the expression of mutable p53 protein in the population at high risk gastric cancer and helicobacter pylori infection

目的:对胃癌高危人群胃粘膜组织中的突变型p53蛋白及Hp进行检测,探讨突变型p53蛋白与Hp感染在胃癌中的关系。方法:利用免疫组化法(S-P)检测178例胃癌高危人群胃粘膜活检标本中突变型p53蛋白的表达情况,并用HE染色和特殊染色检测Hp感染情况。结果:178例胃粘膜组织中,突变型p53蛋白在102例慢性浅表性胃炎(CSG)中的阳性表达率为4.90%,在76例慢性萎缩性胃炎(CAG)中的阳性表达率为25.00%,在伴有肠上皮化生(IM)的60例中的阳性率为40.00%,在伴有异型增生(DYS)的9例中阳性表达率为55.55%,随着病变的加重阳性表达率有增高趋势(P<0.005)。Hp在CSG中的感染率为40.19%,在CAG中的感染率为65.78%,在伴IM中的感染率为66.67%,在伴DYS中的感染率为77.78%,随着病变的进展Hp的感染率有逐渐升高的趋势,(P<0.005)。91例Hp阳性组中突变型p53蛋白的阳性表达率为20.87%,87例Hp阴性组中突变型p53蛋白的阳性表达率为5.74%,二者有显著性差异(P<0.005)。结论:p53基因的突变和Hp感染是陕北胃癌高发的重要因素,可能有协同致癌作用。

Objective： To investigate the relationship between the expression of mutable p53 protein in the population at gastric cancer risk and Helicobacter pylori infection. Methods： The immunohistochemical staining technique （S- P method） was used to detect the expressions of p53 in 178 specimen of gastric cancer. Hp was detected by staining with HE and special staining. Results： The protein expression rate of mutable p53 in CSG, CAG, IM, DYS were 4.09%, 25.00% ,40.00% and 55.55% respectively. With the lesion＇s progress, the expression rate increased （P 〈 0.005 ）. The expression rates of Hp infection in CSG, CAG, IM, and DYS were 40.01%, 65.78% ,66.67% and 77.78% respectively. With the lesion~ progress, the infection rate increased （P 〈0. 005 ）. In the population of high risk gastric cancer, the protein expression of p53 was much higher in the Hp positive group than negative one （ P 〈0.005 ）. Conclusion： Mutable p53 and Hp infection may be the important factors to the gastric cancer at high risk population.