胸腔积液细胞学及免疫细胞化学在鉴别肺转移性腺癌和间皮细胞中的临床价值分析

The clinical significance of hydrothorax cytology and immunocytochemistry in differential diagnosis of metastatic pulmonary adenocarcinoma and mesothelial cells

目的:探讨胸腔积液细胞学及免疫细胞化学在鉴别肺转移性腺癌和间皮源性细胞的临床价值。方法:应用细胞学和细胞块免疫细胞化学方法,检测96例胸腔积液中转移性腺癌和间皮细胞,在细胞学形态上的鉴别及在CEA、CK、TTF-1、KI67、CR、Vimentin中的表达。每例均制备细胞学涂片和细胞块,并用细胞块切片作免疫细胞化学染色。结果:细胞形态学和免疫细胞化学相结合,96例胸腔积液中诊断腺癌42例,间皮细胞54例。结论:细胞学和CEA、CK、TTF-1、KI67、CR、Vimentin6种抗体联合检测,可鉴别诊断肺转移性腺癌细胞和间皮源性的细胞,提高了疑难病例诊断准确率。

Objective：To expore the clinical significance of hydrothorax cytology and immunocytochemistry in differential diagnosis of metostatic pulmonary adenocarcinoma and mesothelial cells. Methods： A total of 96 hydrothorax cytology specimens were tested by HE stainning smears and cell blocks ,immunocytochemical staining was detected for cell block sections for CEA,CK,CR,TTF-1,Ki-67 and Vimentin. Results： Combinaed cytological characteristics with immunocytochemistry staining on 96 cases, 42 cases adenocarcinoma and 54 cases mesothelial cell were diagnosed. Conclusion： Combined detection of CEA,CK TTF-1,Ki-67,CR and Vimentin with cytology is a method for diffentiation between metastatic pulmonary adenocarcinoma and mesothelial cells.