维甲酸对DENA诱发肝癌大鼠的T淋巴细胞亚群与NK细胞的影响

Effects of the T-lymphocyte subsets and NK cells induced by retinoic acid on the hepatocarcinoma in rat

目的:本文探讨维甲酸抑制大鼠肝癌细胞增殖对T淋巴细胞亚群和NK细胞的影响。方法:设三个实验组,分别收集30个大鼠外周血样本,然后进行免疫荧光抗体标记。应用流式细胞仪(EPICS-XL)检测T淋巴细胞亚群和NK细胞的百分率。结果:对照组与健康组比较,CD4+细胞数明显减少,CD8+细胞数明显增多,CD4+/CD8+比值下降(P<0.01)。维甲酸组与健康组比较,CD4+、CD8+和CD56+细胞数均无显著性差异(P>0.05)。结论:维甲酸抑制大鼠肝癌细胞增殖时对机体免疫功能的改善有一定影响。

Objective：To investigate the effects of the T-lymphocyte subsets and NK cells induced by retinoic acid on the hepatocarcinoma in rat.Methods：To establish three experiments groups.After the samples of peripheral blood from 30 rats were stained with fluorescent-conjugated monoclonal antibodies,the percentages of T-lymphocyte subsets and NK cells were detected with flow cytometry（FCM）.Results：The percentage of CD4＋ cells was decreased and CD8＋ cells was increased and the ratio of CD4＋/CD8＋ was lower in the control group compared with the percentage of the healthy groups（P〈0.01）.The percentage of CD4＋ and CD8＋and CD56＋ cells in the retinoic acid groups were not significantly different from the healthy groups（P〉0.05）.Conclusion：The results suggested that rat with the hepatocarcinoma induced by retinoic acid can improve body immune function.