牙本质黏结剂诱导人牙髓细胞凋亡的体外研究

Dental bonding agent induces apoptosis of human dental pulp cells in vitro

目的:比较不同固化时间牙本质黏结剂对牙髓细胞凋亡的诱导作用。方法:体外培养人牙髓细胞,与黏结剂模型共同孵育24h,经PI染色,采用流式细胞记数仪检测凋亡细胞数目。结果:经t检验,与对照组比较,光照0s、10s和20s对牙髓细胞凋亡都有不同程度的影响(P<0.05),光照时间延长可减少对牙髓细胞凋亡的诱导。结论:牙本质黏结剂可诱导牙髓细胞凋亡,适当延长固化时间可以减轻黏结剂对牙髓的刺激。

AIM： To compare the effect of different light curing time of dental bonding agent to induce the dental pulp cells apopotois in vitro. METHODS ： Dental pulp cells were cultured in vitro. The pulp cells were exposed to agents for 24 hours. Propidium iodide staining followed by flow cytometry was used to evaluate the effects of dental bonding agent on cell apoptosis. RESULTS ： By t - test, light curing for 0,10 - 20 seconds could induce apoptosis （ P 〈 0.05）. The longer the light curing time of agent was, the slighter the apopotosis of dental pulp call was induced. CONCLUSION： Dental bonding agent induces apoptosis of dental pulp. Prolonging the light curing time can reduce the stimulation of dental agent on pulp.