伞房花耳草中乌索酸的分离提纯及HPLC分析

Separation and HPLC Analysis of Ursolic Acid in Hedyotidis Corymbosae Lam

采用"醇提凝析法"从伞房花耳草中分离提纯乌索酸,并用光谱等技术对乌索酸结构进行了表征,建立了伞房花耳草中乌索酸的分离提纯及含量测定的方法。结果表明,此方法具有快速简便、精密度高、重现性好、线性范围宽的特点,可用于乌索酸的质量控制。

To develop a method for extracting and analysis of ursolic acid in Hedyotidis Corymbosae Lam.the method of ethanol extracting and agglutination separating were used to extract ursolic acid from Hedyotidis Corymbosae Lam and the structure of ursolic acid was identified by IR,MS,NMR.HPLC was carried out on the chromatographic column of SymmetryShield RP18（3.9 mm×150 mm,5 μm）at 25 ℃,using methanol-water（88∶12,V/V）as mobile phase with a flow of 1.0 ml/min,and detection wavelength was set at 210 nm.There was a good relationship between the peak area and the sample content injected at the range of 3.9～19.6 μg（r = 0.999 9）.The content was 99.58% by HPLC and the gain rate was 3.04‰.The method was simple,accurate and had good repeatability and wider linear range,and can be used to evaluate the quality of ursolic acid.