多菌灵降解菌株的分离以及降解条件研究

Research on the Isolation and Degradation Conditions of Strain Degrading Carbendazim

[目的]研究菌株降解多菌灵的条件。[方法]用富集培养法,分离出1株降解多菌灵的细菌,对其降解效能及特性进行研究。[结果]该菌株为假单胞菌属,5 d内对100 mg/L多菌灵的降解率为61%,能够以多菌灵为碳源进行生长。25-35℃内菌株对多菌灵的降解较好。菌株对多菌灵的降解在pH值5.0-8.0内相差不大。随着接种量的增大降解率增加,接种量为10%时最大。随着碳源、氮源的加入降解率增加,碳源加葡萄糖降解率最大为86%,氮源加0.5%蛋白胨降解率最大,5 d后对多菌灵的降解率达90%。多菌灵浓度较低时,菌体的生长量随培养时间的延长而增加;多菌灵浓度较高时,菌体的生长表现出先缓慢增加后减小的趋势。[结论]pH值7.0、培养温度30℃、接种量10%、0.5%蛋白胨碳源为该菌株降解多菌灵的最佳条件。

The aim was to study the condition of strain degrading carbendazim.1 strain of bacteria degrading carbendazim was isolated by enrichment culture method and its degradation efficiency and characteristics were studied.The strain belonged to Pseudomonas spp.with degradation rate of 61% on 100 mg/L carbendazim in 5 days,and could grow with carbendazim as carbon source.The degradation of the strain on carbendazim at 25～35 ℃ was better.The degradation of the strain on carbendazim at pH value of 5.0～8.0 had little difference.The degradation rate increased along with the increment of incubation amount,and was most when the incubation amount was 10%.The degradation rate increased along with the addition of carbon and nitrogen source and the most degradation rate with glucose as carbon source was 86%,and that with 0.5% protein peptone as nitrogen source was most,reaching 90% on carbendazim after 5 days.When the concentration of carbendazim was lower,the growth amount of bacteria increased along with the prolonging of culture time.When the concentration of carbendazim was higher,the growth of bacteria was slowly increased at first,and then was decreased.The optimum condition of the strain degrading carbendazim was pH value of 7.0,culture temperature of 30 ℃,incubation amount of 10%,with 0.5% protein peptone as carbon sources.