大花蕙兰组织培养与快繁技术

Tissue Culture and Rapid Propagation Technologies of Cymbidium grandiflorium

[目的]研究大花蕙兰组织培养与快繁技术,寻求最佳培养基。[方法]以大花蕙兰的原球茎为接种材料,以MS为基本培养基,设计不同生长调节物质浓度的组合、不同激素配比,探讨了不同培养基对大花蕙兰原球茎增殖、诱导芽及生根的影响和不同激素配比对形成根的影响。[结果]大花蕙兰组织培养原球茎增殖以MS+0.1 mg/L NAA+2.0 mg/L 6-BA培养基配方最好;适合大花蕙兰组织培养原球茎诱导分化的培养基配方为MS+1.5 mg/L 6-BA+0.3 mg/L NAA,诱导出芽率可达130%;适合大花蕙兰诱导生根的培养基配方为1/2 MS+0.2 mg/L NAA+1.0 mg/L 6-BA,试管苗移栽后成活率为78%。[结论]6-BA浓度的增加,可明显提高原球茎的增殖,高浓度6-BA与低浓度NAA的配比较适合大花蕙兰的原球茎增殖;6-BA在1.5 mg/L时最有利于原球茎的诱导分化。

[Objective] The research aimed to study the tissue culture and rapid propagation technique of Cymbidium grandiflorium and seek the optimum medium.[Method] With the protocorms of C.grandiflorium as inoculated materials and MS as basic medium,different combinations of growth regulating substances with different concentrations and different hormone proportions were designed to discuss the effects of different medium on the protocorm propagation,bud induction and rooting of C.grandiflorium and the effects of different hormone matching on the formation of roots.[Result] The optimum medium formula for protocorm propagation in tissue culture of C.grandiflorium was MS ＋0.1 mg/L NAA ＋ 2.0 mg/L 6-BA.The suitable medium for protocorm induction and differentiation in tissue culture of C.grandiflorium was MS＋1.5 mg/L 6BA＋0.3 mg/L NAA with the bud-inducing rate up to 130%.The suitable medium for root inducement was 1/2MS＋0.2 mg/L NAA ＋1.0 mg/L 6-BA and the survival rate of in vitro plantlets after transplanting was 78%.[Conclusion] The increment of 6-BA concentration can increase the protocorm propagation obviously.And the matching of high concentration of 6-BA and low concentration of NAA is more suitable for the protocorm propagation of C.grandiflorium.6-BA at the concentration of 1.5 mg/L was most favorable for protocorm induction and differentiation.