摘要
对流苏石斛茎段的添组织培养进行了初步研究。结果表明,在诱导茎段腋芽萌发中,以MS+6-BA4.0 mg/L+NAA1.0 mg/L效果最好;在芽增殖阶段,以MS+6-BA2.0 mg/L+NAA0.1 mg/L增殖效果最佳;在研究附加物对无菌苗增殖影响时,添加15%的椰乳可有效提高芽的增殖率。
The stem segments of D.fimbrialum Hook used as explants were studied.The results showed that: MS+6-BA 4.0 mg/L+NAA 1.0 mg/L was optimal for bud induction,MS+6-BA 2.0 mg/L+NAA 0.1 mg/L was the best for bud multiplication.While studying the influence of the complement on the seeding multiplication,it showed that adding 15% coconut juice can get the best effect of enhancing the coefficient of the multiplication.
出处
《安徽农业科学》
CAS
北大核心
2007年第33期10704-10706,共3页
Journal of Anhui Agricultural Sciences
基金
广西教育厅科技项目
玉林师范学院博士启动项目
关键词
流苏石斛
茎段
组织培养
D.fimbrialum Hook.
Stem segments
Tissue Culture