发酵法生产透明质酸工艺研究

Process research on producing hyaluronic acid by fermentation method

研究了兽疫链球菌直接上发酵罐生产透明质酸的条件,考察培养基配方、温度、通气量、pH值、葡萄糖浓度等对产物得率的影响,获得了较佳的培养基配方:葡萄糖70 g/L、酵母粉25 g/L、硫酸镁5 g/L、磷酸二氢钠5 g/L、维生素B11.0g/L,较佳发酵工艺条件为:培养温度为36±1℃、通气量为2.0 vvm、pH值为6.80±0.1.在此条件下透明质酸的产率为7.06 g/L.研究结果表明,直接上发酵罐生产透明质酸的方法,不仅减轻了劳动强度,而且节省了种子罐的生产环节,减轻了染菌机率。

The effects of culture and fermentation conditions on the fermentation of hyaluronic acid （HA） produced by Streptococcus zooepidemicus were studied. The optimum medium in culture was obtained through experiment ： glucose 70g/L, yeast powder 25g/L, MgSO4 5g/L, NaH2PO4 5g/L, vitamin B1 1.0g/L, and the optimum fermentation Conditions were： temperature 36 ± 1℃, aerobic rate 2.0vvm, pH = 6.80 ± 0.1. Under the optimum condition, the HA yield reached 7.06g/L.