S-诱抗素的高效液相色谱分析

Quantitative Analysis of S-ABA by HPLC

本文介绍了S-诱抗素的反相高效液相色谱定量分析方法。样品溶于甲醇中,采用二极管阵列检测器和Agilent chiradex 250mm×4mm(i.d),5μm手性柱;以甲醇:纯水(60:40,v/v)作为流动相,流速为0.8mL/min。该方法平均回收率为99.52%。线性相关系数、变异系数和标准偏差分别为:1、2.27%和0.028。

A RP-HPLC method for the analysis of S-ABA was described,Agilent ChiraDex 250mm×4mm （i.d） ,5μm chirality column and DAD detector at 260 nm were used,The sample was dissolved in methanol and the mobile phase was methanol/water （60/40,V/V） .The recoveries ranged from 98.49%-101.3%.The correlation coefficient was 1.000,the coefficient of variation and the standard deviation were 2.27% and 0.028, respectively.