棉铃虫抗药性的生理生化机制研究

BIOCHEMICAL AND PHYSIOLOGICAL MECHANISMS OF INSECTICIDE RESISTANCE IN HELICOVERPA ARMIGERA (HBNER)

本文报道了棉铃虫Helitoverpaarmigera田间抗性种群对杀虫剂抗药性的生理生化机制。抗性种群（HJ－R）5龄幼虫羧酸酯酶、谷胱甘肽转移酶、多功能氧化酶活力均明显高于相对敏感种群（HD－S）。两种群乙酰胆碱酯酶对杀虫剂敏感性没有显著差异。HJ－R种群的腹神经索对氰戊菊酯表现了2～3倍的神经不敏感性。HJ－R种群对氨基甲酸酯类杀虫剂的抗性主要是由代谢机制引起，其中多功能氧化酶可能起主导作用；对菊酯的抗性是由多功能氧化酶、酯酶、以及神经不敏感性几个因子综合作用的结果。

Mechanisms of insecticide resistance were studied in a field-selected resistant population (HJ-R) of Helicoverpa armigera. The fifth instar larvae of HJ-R strain possessed higher activity of esterase、glutathion S-transferase、monooxygenase than that of susceptible strain (HD-S). There was no significant difference between the two strains in sensitivity of acetylcholinesterase to insecticide. The central nervous system of HJ-R strain showed 2~3 time more insensitive to the action of fenvelerate. The metabolic detoxication was the main reason of resistance to organophosphorus and carbamate insecticides. The monooxygenase seems to be the most important mechanism of carbamate resistance in HJ-R population. The resistance to pyrethroids were caused by both target insensitivity and metabolic detoxication of monooxygenase and esterase.