黄瓜与小麦和大豆轮作对土壤微生物群落物种多样性的影响

Effect of Soybean-Cucumber and Wheat-Cucumber Rotation on Soil Microbial Community Species Diversity

采用黄瓜与小麦和大豆轮作的方式,研究轮作对黄瓜土壤微生物群落DNA序列多样性的影响。结果表明,黄瓜与小麦和大豆轮作显著提高了土壤微生物多样性指数、丰富度指数和均匀度指数,两种轮作土壤微生物群落DNA序列相似程度高达0.5678,而与对照土壤间的相似程度分别为0.3465和0.3124。两种轮作处理的黄瓜产量也显著高于对照(P<0.05)。说明轮作改善了土壤的微生态环境。

Effects of soybean- cucumber and wheat -cucumber rotation on soil microbial community DNA sequence diversity were evaluated using random amplified polymorphic DNA （RAPD） method. The resuhs showed that grain-cucumber rotation significantly increased the diversity index, richness index and evenness index of soil microbial community, and the soybean - cucumber rotation was more effective than wheat - cucumber rotation in terms of the microbial community. Soil microbial community DNA sequence similarity coefficient between the two rotation soils was 0. 5678 ; However the similarity coefficients between the two rotation soils and control soil were 0. 3465 and 0. 3124, respectively. Cucumber yields in two rotation soils were also higher than that in control soil significantly. This may imply that rotation can improve soil microbial ecology.