应用大孔吸附树脂分离纯化香椿叶总黄酮

Separation and Purification of Total Flavonoids from Toona sinensis Leaves by Macroporous Resins

测定了6种大孔吸附树脂对香椿叶中总黄酮的吸附解吸性能,确定了大孔吸附树脂吸附分离香椿叶总黄酮的工艺条件。结果表明LSA-10树脂对香椿叶总黄酮有良好的吸附解吸性能,其最佳吸附分离的工艺条件为:上柱液总黄酮质量浓度0.653mg/mL,pH4.0～5.5,流速240mL/h,饱和吸附量440～480mL,用400mL50%乙醇以流速80mL/h解吸。经4次上柱吸附解吸,香椿叶总黄酮纯度可达75.2%。

In order to establish the technology for separating and purifying total flavonoids from Toona sinensis leaves with macroporous resin, 6 types of macroporous resins were selected to be investigated. The resuits showed that LSA-10 was the best for separating and purifying total flavonoids in the following conditions： the loaded concentration of total flavones 0. 653 mg/mL, pH 4. 0 -5.5, the flow rate 240 mL/h; amount of saturated adsorption for total flavones 440 -480 mL; 400 mL 50% ethanol as appropriate the eluting reagent, eluting rate 80 mL/h. The purity of total flavonoids purified by LSA-10 was 75.2% when purified four times under the optimal conditions.