转基因产品免疫学检测技术研究进展

Immunoassay Methods for Detection of Genetically Modified Organisms in Foods

目前转基因产品的检测主要有基于DNA的检测方法以及基于蛋白质的免疫学检测方法,简述了免疫学检测方法中的免疫印迹法(Western blotting)、酶联免疫吸附试验(ELISA)和试纸条法的原理、特点、应用例子以及存在问题。对一些新的方法如免疫PCR法、生物传感器法也略作介绍。

At present there are two kinds of methods to measure transgenic products：measuring the DNA and measuring the protein （immunodiagnostic methods）. Some kinds of immunodiagnostic methods were reviewed in this article,including Western Blotting、Enzyme-Linked Immunosorbent Assay and Lateral Flow Strip. Discussions were made in terms of their principle,characteristic,application and problems. Several new methods such as Immuno-PCR,Biosensors were also summarized in this article.