低温联合紫外照射增强苦参碱诱导Colo320细胞凋亡作用的研究

The Study on Low Temperature unite Ultraviolet Irradiation' s Effect of Enhancing Matrine Induces Apoptosis in the Colo320 Tumour Cell

目的从细胞凋亡角度探讨低温、辐射、中药综合治疗大肠癌的机制。方法采用Hoechst33258荧光染色技术,检测低温、紫外线两种物理因素及中药苦参碱分别及联合运用时的Colo320细胞凋亡率。结果倒置显微镜下均可以观察到细胞凋亡情况。经单纯低温-4℃,0℃,4℃处理,凋亡率为30.98%、31.72%、33.06%;经单纯15W紫外灯下50、100、150cm处照射,凋亡率为35.56%,22.69%,15.08%;经低温与紫外照射共同影响,其凋亡率为42.31%;经低温联合紫外照射及苦参碱共同作用,凋亡率达48.17%。结论Co-lo320细胞凋亡反应在低温、紫外照射及苦参碱的共同影响下,其诱导凋亡作用明显提高。

Objective： To research the mechanism of low temperature, irradiation and Chinese traditional medicine treating the colorectal cancer from the perspective of apoptosis. Methods： Hoechst 33258 fluorescent staining detected the colo320 tumour cell＇ s respective and jointed apoptosis rate by two physical factors of low temperature and ultraviolet irradiation combining matrine. Results： Each apoptosis situation could be inspected through the inverted microscope. After the low temperature about -4℃ , 0℃ and 4℃ , the apoptosis rates were 30. 98%, 31.72%, 33.06%. After the 15W ultraviolet irradiation in the 50, 100, 150cm position, the apoptosis rates were 35.56%, 22.69%, 15.08%. After low temperature and ultraviolet irradiation, it was 42.31% ; After low temperature unite ultraviolet irradiation and the matrine, it reached 48. 17% Conclusion： The Colo320 cell＇ s apoptosis rate is significantly increasing responds to low temperature unite uhraviolet irradiation and matfine.