肌细胞分化基因与大鼠肝再生的相关性分析(英文)

EXPRESSION PROFILES OF THE GENES ASSOCIATED WITH THE MYOCYTE DIFFERENTIATION DURING RAT LIVER REGENERATION

肌细胞是组织器官的重要组成部分。为在基因转录水平了解肌细胞分化相关基因在大鼠肝再生中的作用.本文用搜集网站资料和查阅相关论文等方法获得上述基因,用Rat Genome 230 2.0芯片检测它们在大鼠肝再生(liver regeneration,LR)中表达情况,用比较真、假手术基因表达的差异性方法确定肝再生相关基因。初步证实上述基因中52个基因与肝再生相关。根据肝再生中基因表达的时间相关性将上述基因聚合为0.5-1h:2-12h;16、30、42、96h;18-24、36、48-60h;66-72、120- 168h等5类,表达上调和下调的基因数分别为8和10,24和8,21和24,53和64,28和36。它们表达的相似性分为均上调、上调占优势、均下调、下调占优势、上调和下调次数相近等5类,涉及15、10、17、7和3个基因,共上调表达143次、下调136次,分为8类表达方式。表明肌细胞分化相关基因表达变化多样和复杂。根据上述结果推测,肝再生中成肌细胞和平滑肌细胞分化增强;骨骼肌和心肌细胞分化相关基因参与肝再生的生理生化活动。

Myocytes are important parts of tissues and organs. To study the effects of myocyte differentiation-related genes on rat liver regeneration （LR） at transcriptional level, we obtained these genes through collecting the database data and retrieving the pertinent thesis, and detected the expression profiles of above-mentioned genes during LR using the Rat Genome 230 2.0 array. LR-associated genes were identified by comparing the discrepancy in gene expression changes between partial hepatectomy （PH） group and sham-operation （SO） group, by which 52 LR-associated genes were confirmed. They were classified into 5 groups based on time relevance, including 0.5-1h; 2-12h; 16h,30h,42h,96h; 18-24h,36h,48-60h; 66-72h,120-168h, in bers of up-regulation and down-regulation genes were 8 and 10, 24 and 8, 21 which the numand 24, 53 and 64, 28 and 36, respectively. Among these genes, the total 143 times of up-regulation and 136 times of down-regulation, as well as their 8 expression patterns displayed diversity and complexity of the genes associated with the myocyte differentiation. It was inferred that the differentiation of myoblasts and smooth muscle cells were enhanced during LR and the genes associated with the differentiation of skeletal muscle cells and cardiac muscle cells participated in the cellular physiological and biochemical activities of LR.