摘要
根据GenBank中鹅生长激素受体(GHR)基因序列设计合成了引物和探针,对荧光定量PCR的方法进行了方法学的评估,建立了TaqmanMGB荧光定量RT-PCR检测方法。结果表明,由pMD-18T-GHR所构建的标准曲线线性关系良好,建立的GHR基因荧光定量PCR检测方法灵敏度高、特异性强(可以检测出低于10个拷贝/μl的样品),准确可靠。籽鹅和莱茵鹅GHRmRNA出壳到90日龄生长过程中肝脏中的表达量不同,30日龄不同组织的表达量各有变化特点。
The probe and primers were designed and synthesized according to the growth hormone receptor sequence of goose available in GenBank. Then a real-time RT-PCR assay was developed and assessed. Resuits showed that the standard curve made by pMD-18T-GHR had good linear dependence, and the fluorescent quantitative RT-PCR assay was sensitive, specific and reliable. The expression of GHR mRNA were difference in liver during postnatal 90 days , and there was different changing pattern in other tissues at 30 days between Zigeese and Rhine.
出处
《中国农学通报》
CSCD
2007年第12期32-36,共5页
Chinese Agricultural Science Bulletin
基金
黑龙江省教育厅资助项目"籽鹅早期生长发育及生长轴部分基因表达规律的研究"(11513070)
大庆市科技局科技攻关项目"大庆市大鹅产业化关键技术的研究"(SGG04-077)。
