摘要
利用RAPD标记技术对19份克新系列马铃薯品种进行了遗传多样性分析,分别提取19份马铃薯品种的DNA,进行随机引物多态性扩增,从1000条随机引物中初步筛选出7条有多态性的引物进行详细研究,每条RAPD引物扩增出4～9条带,共获得52条带,其中多态性条带为43条;19份马铃薯品种的遗传距离介于0.17～0.72之间,平均值为0.39,平均遗传距离介于0.31～0.51之间;聚类分析结果在GS=0.53处可将克新系列马铃薯品种划分为三类,聚类结果与系谱分析基本相符,同时也说明克新系列马铃薯的遗传基础有所拓宽。研究表明:RAPD标记简便、快速、成本低,适用于分析马铃薯遗传多样性,指导马铃薯育种实践中的亲本组配。
The RAPD was used to study the genetic diversity of 19 KinXin series potato cultivars. The result showed that 52 RAPD bands were amplified using 7 primer, among them 43 bands were polymorphie, and 4-9 bands were amplified in each lane. The RAPD analysis indicated that the genetic distance of 19 potato cuhivars was between 0.17-0.72, average value was 0.39. When all of the polymorphie bands were analysed by cluster analysis, 19 potato euhivars were classified into 3 groups. The analysis showed that there existed close sibship among the majority of potato cultivars, and the genetic distance between the minority cultivars and the others is longer, which indicate that the genetic basis of KeXin series potato euhivars is wide. The RAPD is more suitable in the diversity study of potato eultivars, and suitable to analyse the sibship of potato eultivars in breeding.
出处
《中国农学通报》
CSCD
2007年第12期81-84,共4页
Chinese Agricultural Science Bulletin
基金
黑龙江省农业科学院科研计划项目"多抗高产专用马铃薯种质资源创新技术研究与应用"(HNY2004113)。
