水分胁迫对冬小麦抗性物质可溶性糖与脯氨酸的影响

The Impact on Soluble Sugar and Proline Contents under Different Water Stress

利用盆栽方法,通过人工调控土壤含水率,设置轻度T1(相对含水率65%～70%)、中度T2(相对含水率55%～60%)和重度T3(相对含水率45%～50%)三个水分胁迫水平,研究不同生育期水分胁迫对冬小麦脯氨酸和可溶性糖含量的变化。通过测定与分析,认为冬小麦旗叶可溶性糖和脯氨酸含量的变化与水分胁迫密切相关,是保障冬小麦在干旱条件下正常生长的抗性物质。轻度水分胁迫对可溶性糖的影响主要集中在生长前期,中度和重度水分胁迫全生育期可显著提高可溶性糖含量,特别是乳熟期,中度和重度水分处理分别比CK增加78.6%和144.0%,因此可溶性糖可能是对种子成熟有特别作用的抗性物质。水分胁迫加强游离脯氨酸含量显著增加,说明脯氨酸也是重要的抵抗干旱的活性物质。在冬小麦全生育期中,开花期脯氨酸含量比正常含量高1倍左右,说明脯氨酸可能是保障正常开花的活性物质。

In this paper, the effect of proline and soluble sugar on water stress is studied. Soil moisture is controlled with pot. Meanwhile, there are three water stress treatment and a contrast treatment： the control treatment （CK, the soil relative moisture is 75%-80%）, mild water stress （T1, the soil relative moisture is 65%-70%）, moderate water stress （T2, the soil relative moisture is 55%-60%）, severe water stress （T3, the relative soil moisture is 45%-50%）. It is proved that proline and soluble sugar of the flag leaf are both resistant materials for winter wheat in dry condition. The soluble sugar content was increased significantly from jointing stage to flowering stage under mild water stress, but it did not increased significantly from grout filling stage to milky stage. Moderate and severe water stress increase the soluble sugar content in the whole life of wheat, and the contents of milk stage increase 78.6%（ moderate water stress） and 144.0%（severe water stress） compare to CK. It shows that soluble sugar might be resistant material with special role for seeds maturing. Praline content increase significantly with the water stress strengthen, it shows that the material is an active substance to resistance drought for winter wheat. The proline contents of flowering stage are about 100% higher than the other four stages＇, it shows that proline is the active substances for the wheat to guarantee flowering.