实验性传染性法氏囊病凝血功能动态研究

STUDIES ON BLOOD COAGULATION DYNAMICS OF EXPERIMENTAL INFECTIOUS BURSAL DISEASE

９０只健康白来航雏鸡于５０日龄接种传染性法氏囊病野毒强化株。分别于攻毒前（即攻毒０ｈ），攻毒后２４ｈ、４８ｈ和７２ｈ随机抽取５只鸡，心脏采血，对其６项凝血指标进行动态测定。结果表明，攻毒后２４ｈ血浆再钙化时间和凝血酶原时间显著延长（Ｐ＜０．００１）；４８ｈ不凝；７２ｈ逐渐恢复。说明传染性法氏囊病伴有严重的凝血功能障碍。此凝血障碍早于腹泻等症状，并呈障碍－严重障碍－逐渐恢复的动态过程。攻毒后２４ｈ和４８ｈ凝血酶时间显著延长（Ｐ＜０．００１），而纤维蛋白原含量无明显差异，表明传染性法氏囊病发病过程中抗凝活性增强。游离肝素时间试验和纤维蛋白（原）降解产物的定性测定进一步说明抗凝活性的增强与肝素无关，而与纤维蛋白（原）降解产物的产生有一定关系。

Healthy White Leghorn chickens were inoculated at postatch day 50 with a field strain of infectious bursal disease virus.Blood samples were collected via heart puncture for determination of 6 coagulation tests at postinoculation hour(PIH)0,24,48 and 72.Plasma recalcification time and prothrombin time were significantly prolonged at PIH 24,showed no end points at PIH 48 and began to return to normal at PIH 72.Thrombin time was significantly longer at PIH 24 and 48 than that at PIH 0.There were no significantly changes in fibirnogen at different times and there were significant increases in fibrin/fibrinogen degradation products at PIH 48.The results showed that there existed severe coagulation dificiency and higher anticoagulative activity in infectious bursal disease,which presented the dynamic changes accordant with clinical process.The comprehensive analysis of results led to conclusion that coagulation dificiency not only cased hemorrhage but also got involved in the other pathogenic processes of infectious bursal disease.