分解蔗渣的研究Ⅱ.特异青霉YB-7纤维素酶的性质研究

STUDIES ON THE BAGASSE CELLULOLYSIS Ⅱ. STUDIES ON THE PROPERTIES OF CELLULASE IN Penicillium notatum YB7

对特异青霉（Ｐ．ｎｏｔａｔｕｍ）ＹＢ－７纤维素酶的一般酶学性质进行了研究。其最适酶作用条件为：ｐＨ５．０，５０℃，且在小于７０℃和ｐＨ４．０～９．０的范围内具有稳定性，ＣＭＣ、ＦＰＡ和β－葡萄糖苷酶活力变化范围有一定差别。甘油、葡萄糖和纤维二糖的最低抑制浓度（ｍｍｏｌ／Ｌ）分别为３．２５、２．４５和１．７１，其抑制系数分别为１．０１，２．３１和２．８０。８０％饱和度的硫酸铵可较完全地沉淀纤维素酶的有效成份。

The general properties of cellulase in P.notatum YB7 was studied. The results indicated that the optimal conditions of enzymtic hydrolysis was at pH 5.0、50℃, cellulase was stable when the pH value was 4.0～9.0 and the tempture was below 70℃, and the courses of CMCase、FPA、βglucosidase were different from each other. The experimental results also showed that the lowest inhibitor concentration of glycerine, glucose and cellobiose was seperately 3.25, 2.45 and 1.71 m molL, and their inhibiting coefficients were 1.01, 2.21 and 2.80, respectively. 80% saturated (NH 4) 2SO 4 could isolate most of the components of the cellulase.