摘要
目的了解受体相互作用相关蛋白(RIP)140在正常小鼠脑发育时期的表达情况。方法应用免疫组化方法研究 RIP140在脑组织中的定位,并应用实时定量 PCR 和 Western 印迹研究RIP140在正常小鼠不同发育时期的动态表达模式。结果免疫组化结果显示 RIP140在正常小鼠脑发育过程中始终存在表达,且在大脑皮质、海马、丘脑等部位有明确的表达。实时定量 PCR 结果显示RIP140 mRNA 在正常小鼠脑不同发育时期的表达呈现动态变化模式,Western 印迹结果示 RIP140蛋白在小鼠脑不同发育时期的表达也呈现动态变化模式,且与 mRNA 的表达具有正相关性(r_s=0.767,P=0.016<双侧>)。结论 RIP140参与了正常小鼠脑发育和脑功能的行使。
Objective To investigate the expression of receptor-interacting protein of 140 000 (RIP140) in the developmental brain. Methods The brain tissues of 15-day-old and 20-day-old fetal Balb/ c mice and 1,7, 14, 28, 42, and 56-day-old postnatal mice were collected. The expression of RIP140 was examined by immunohistochemistry. Real-time PCR and Western blotting were used to quantify the expression of the mRNA and protein levels of RIP140. Results (1) Immunochemistry showed that RIP140 was expressed extensively in the brain of embryonic and newborn mice, mainly present in the neurons in many different brain regions, such as the cerebral cortex, hippocampus and pituitary gland. The cellular location of RIP140 was confined to the nucleus. (2) Real-time PCR revealed that the RIP140 mRNA expression in brain was in an increment as the time went by, peaked on the 7th day after birth, then was in a instable level after that until the adulthood. (3) Western blotting indicated that the protein level of RIP140 was coincident with the mRNA level [ rs = 0.767, P = 0. 016 (bilateral) ]. Conclusion The expression and role of RIP140 may be involved in the whole neurodevelopment stages of brain, and may take part in the development and the function of the brain.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2007年第44期3152-3154,共3页
National Medical Journal of China
基金
国家重大基础研究发展规划基金(2001CB510303)