益母草与川芎配伍水煎醇沉工艺实验研究

Experimental Research in Decoction and Alcohol Sedimentation Technique of Herba Leonuri Combined with Rhizoma Chuanxiong

目的:优选肾宁颗粒制备中益母草与川芎的最佳水煎醇沉工艺。方法:采用正交试验法。以盐酸水苏碱含量和干膏收率为指标,对影响提取的各因素进行考察;以水苏碱含量和80%乙醇浸出物得率为指标,对影响醇沉的各因素进行考察。结果:最佳工艺为煎煮2次,每次2h,每次加水12倍量;水提液浓缩至1:1.5(g:ml),加乙醇使含醇量达50%,静置48h。结论:所建立提取纯化工艺稳定,为肾宁颗粒及其他制剂的生产提供了参考。

Objective： To optimize the decoction and alcohol sedimentation technique of Herba leonuri combined with Rhizoma Chuanxiong in shenning granules. Method： Orthogonal test was employed. The conditions for decoction were studied by the dried water deeoeting rates and contents of staehydrine hydroehloride in the extract; the conditions for alcohol sedimentation were studied by the extraction rate of 80% alcohol and contents of staehydrine hydroehloride. Result： The optimal conditions were as follows： to boil with water for two times, sustaining for two hours and 12 times volume of water was used for each time, to concentrate the solution of waterextraction to 1：1.5 （g： ml） and then precipitate in 50% alcohol for 48 hours. Conclusion： The optimal process is stable; it can provide the reference for the preparation of Shenning Granules and other preparations.