摘要
目的:建立辛芩口服液中木兰脂素含量的RP-HPLC测定方法。方法:采用Shim-pack VP-ODS色谱柱C_(18)(250mm×4.6mm,5μm);流动相为乙腈-四氢呋喃-水(35:2:63);流速:1.0ml·min^(-1);检测波长:278nm;柱温:室温.结果:木兰脂素在32～320μg·ml^(-1)范围内线性关系良好(r=0.9999),平均回收率为100.4%,RSD为0.8%(n=6).结论:该方法简便、灵敏、重现性好。
Objective： To establish an RP-HPLC method for determination of Magnolin in Xinqin oral Solution. Method： Shimpack VP-ODS column （250 mm×4.6 mm,5 μm） was used, the mobile phase was acetonitrile- tetrahydrofuran - water（35：2：63 ） ;the flow rate was 1.0 ml·min^-1 ;The detection wavelength was at 278 nm and column temperature at 25 ℃. Result： The linear range of Magnolin was 32 - 320 μg· ml^-2 （ r = 0. 999 9 ）, The average recovery was 100.4%, RSD = 0.8% （ n = 6）. Conclusion： The method is simple, sensitive and reproducibility.
出处
《中国药师》
CAS
2007年第12期1218-1220,共3页
China Pharmacist
