粒细胞集落刺激因子动员骨髓干细胞治疗大鼠急性脑梗死

Effects of granulocyte-colony stimulating factor mobilizing bone marrow stem cell on treatment of acute ischemic cerebral infarction in rats

目的：探讨粒细胞集落刺激因子（G-CSF）对大鼠脑梗死的治疗效果。方法：采用线栓法制备大鼠脑梗死模型,于术后24 h进行神经病学评分分级,入选2分及以上的大鼠共20只,被随机分为给药组和对照组,每组10只。给药组腹腔注射G-CSF 10μg·kg^-1·d^-1;对照组腹腔内注射等量生理盐水均连用10 d。两组分别均于术前、术后10、20和27 d进行神经病学评分分级,于术前及术后10 d和27 d进行体重测定,并计算体重减轻率。术后27 d,将大鼠麻醉后处死取脑,进行氯化三苯四唑（TTC）染色,计算脑梗死容积与全脑容积比值。结果：术前两组体重比较差异无统计学意义（P〉0.05）;术后10 d和27 d,给药组大鼠体重减轻率显著小于对照组〔（-13.15±0.05）%比（-16.46±0.06）%,（9.72±0.10）%比（-8.97±0.05）%,P均〈0.01〕;术后10 d,两组神经病学分级评分比较差异无统计学意义（P〉0.05）;术后20 d和27 d,给药组神经病学分级评分〔（1.30±0.48）分和（0.60±0.52）分〕显著低于对照组〔（2.20±0.92）分和（1.70±0.68）分,P〈0.05和P〈0.01〕;术后27 d,给药组脑梗死容积与全脑容积比值显著小于对照组〔（12.99±3.55）%比（22.87±2.12）%,P〈0.01〕。结论：脑梗死急性期1～10 d给予G-CSF可以有效减轻大鼠缺血性脑梗死程度,减少脑梗死体积,促进大脑神经功能的恢复。

Objective： To approach the therapeutic effect of granulocyte-colony stimulating factor（G-CSF） for treatment of rats with ischemic cerebral infarction.Methods： Twenty-four hours after ligating the left middle cerebral artery（MCA） and left common carotid artery（CCA） to induce cerebral infarction,Zeal Longa＇s neurological behavioral assessments were performed.There were 20 SD rats with scores equal to or more than 2 scores,and they were divided randomly into the G-CSF treatment group and the control group（each n=10）.The G-CSF treatment group received daily abdominal cavity injection of G-CSF 10 μg/kg from 1st day to 10th day;the control group received daily abdominal cavity injection of the same volume of saline from 1st day to 10th day.In the two groups,neurological behavioral assessments were performed before operation and 10,20 and 27 days subsequent to the operation;the body weights were measured before operation and 10 and 27 days after the operation and the rate of loss in body weight was counted. Twenty-seven days after the operation,the rats were anesthetized and killed,and their cerebrums were taken out and stained by triphenyl tetrazolium chloride（TTC） to count the percentage of infarct volume to the total volume of the cerebrum.Results： Before ligating arteries,the body weight decrease rates were not significantly different between the two groups（P〉0.05）.On the 10th and the 27th day subsequent to ligation,the body weight decrease rate of the treatment group was much smaller than that of the control group（（-13.15±0.05）% vs.（-16.46±0.06）%;（9.72±0.10）% vs.（-8.97±0.05）%,both P〈0.01）. On the 10th day after operation,the neurological behavioral assessments were not significantly different between the two groups（P〉0.05）.On the 20th day and the 27th day subsequent to ligation,the neurological behavioral assessments of the treatment group （（1.30±0.48） score and（0.60±0.52） score） were much smaller than that of the control group（（2.20±0.92） score and（1.70±0.68） score,P〈0.05 and P〈0.01）.On the 27th day after operation,the ratio of the infarction volume to the total cerebral volume of the treatment group was smaller than that of the control group（（12.99±3.55）% vs.（22.87±2.12）%,P〈0.01）.Conclusion： Daily abdominal cavity injection of G-CSF 10 μg/kg for 10 days in rats with acute ischemic cerebral infarction can relieve the ischemia degree,reduce the infarction volume and promote the recovery of cerebral functions.