BDNF前体蛋白在成年恒河猴中枢神经系统中的分布

DISTRIBUTION OF proBDNF IN CENTRAL NERVOUS SYSTEM OF ADULT RHESUS MONKEY

采用免疫组织化学方法探讨脑源性神经营养因子前体蛋白(proBDNF)在成年恒河猴中枢神经系统(CNS)内的定位分布。结果显示:不同染色强度的proBDNF免疫阳性反应产物广泛分布于大脑皮质、海马、丘脑、小脑、中脑、脑桥、延髓和脊髓。神经元和胶质细胞的核染色明显,胞浆淡染,突起无染色。本研究结果表明在CNS的各个部位均存在proBDNF,提示proBDNF在猴的CNS广泛发挥作用。通过与文献报道的proBDNF在大鼠CNS的分布比较,发现proBDNF在不同物种之间的表达模式和作用可能存在差异;而与BDNF在成年猴脑中的分布进行比较,发现proBDNF在猴小脑的Purkinje细胞层及动眼神经核单独表达,提示proBDNF在这些部位可能发挥重要作用。本研究结果为探讨proBDNF在成年猴的分布及作用提供了重要的形态学资料。

Immunohistochemical technique was used to detect the localization and distribution of proBDNF throughout in the central nervous system （CNS） of adult rhesus monkey. The result showed that varying intensity of proBDNF-immunopositive preduets was distributed widely in the eerebrum eortex, hippocampus, thalamus, eerebellum, midbrain, pons, medulla oblongata and spinal eord. The nuelei were obviously stained, the eytoplasm was more light and no the process was stained in the neurons and gila eell. The present results suggest that proBDNF distributed widely in the many regions of CNS and may play abroad roles in tbe CNS of monkey. After eomparison with that in rat CNS, we found that proBDNF expressed solely in some areas of the primate CNS whieh might attribute to different species; while eomparison with BDNF distribution in adult monkey brain, it showed that proBDNF was solely deteeted in Purkinje cell layer of eerebellum and oculomotor nueleus. It suggests that proBDNF might play an important role in these regions. The present results provide a morphologieal data for elueidating the distribution and funetion of proBDNF in the adult monkey CNS.