应用聚合酶链反应对病毒性心肌炎病毒学诊断的意义

Application of polymerase chain reaction for pathogenic diagnosis of virus myccarditis

为探讨聚合酶链反应（PCR）在柯萨基B组病毒对病毒性心肌炎病原学的诊断意义。对41例诊断为急性病毒性心肌炎的住院病人，用PCR方法检测其血中的柯萨基B组病毒，并对其中29例病人进行了潘生丁99mT2MIBI心肌灌注显像。结果：柯萨基B组病毒的检出率为80.5％，心肌灌注显像发现单一或多部位同位素持续性稀疏表现。本文结果提示：PCR检测柯萨基B病毒特异性强、敏感、快速及简便，对病毒性心肌炎的病原学诊断有重要价值。

Virus myocarditis is the one of the most common diseases of the cardiovascular system among youngadults. The pathogenic diagnosis technics have been sought. In this study, polymerase chain reaction (PCR) was usedfor detecting virus in 41 adults suffering from acute virus myocarditis. The results showed that 85. 5% (33/41) of thepatients were positive for Coxsackievirus B. PCR is a highly specific, sensitive, fast, as well as easy performing technic.In conclusion, PCR is a valuable technic in detecting virus that can be used for pathogenic diagnosis of virus myocarditis.