应用Fura-2/Am检测血小板胞浆游离钙浓度

Measurement of cytoplasmic free Ca^(2+)in human platelet using Fura-2/Am

以新型荧光指示剂Fura-2／Am检测血小板胞浆内游离钙浓度，并探讨检测过程中的某些实验条件。结果表明：正常成人参考值为137．6±15．2nmol／L，95％正常值范围为107．8～167．4nmol／L。批内及批间变异系数CV分别为6.22％、4.6％，重复性较好。且实验不受正常饮食，溶血、黄应及高脂样品的干扰，证实此方法是一种较为简便可靠的检测血小板胞浆游离钙浓度的方法。

We used a new fluorescent indicator fura-2/Am to measure the cytoplasmic free Ca2+ in human plateletsand investigated some factors in the measurement. Our results indicated that the normal adult's value was 137. 6±15.2nmol/L,the normal value of 95% ranges from 107. snmol/L to 167. 4nmol/L. The coefficient of varition (CV) was6. 22% in groups and 4. 6% between groups. The test was repetitive and wasn't affected by normal diet, hemolysis,jaundice and hyperlipidemia, so this test may be a reliable and simple method for measuring cytoplsmic free Ca2+ inplatelets.