人肝及肾组织中β-葡萄糖醛酸酶mRNA的检测及意义

Detection and Significance of β-Glucuronidase mRNA in Human Liver and Kidney Tissues

目的建立新的人肝脏及肾脏组织中β-葡萄糖醛酸酶(β-G)mRNA的检测方法,并进行不同组织间的比较。方法收集10例正常肝脏、10例正常肾脏及8例肝癌组织,采用逆转录-聚合酶链反应(RT-PCR)方法,检测β-G mRNA在各组织中的表达。结果β-G mRNA的基因扩增产物在人肝脏及肾脏正常组织及肝癌组织中均可被检测,且产物大小一致,均为422bp;β-G mRNA相对表达含量在正常肝脏组织(1.71±0.32)和正常肾脏组织(1.83±0.22)间比较,差异无统计学意义(P>0.05);而正常肝脏组织与肝癌组织(3.88±0.86)比较,差异有统计学意义(P<0.01)。结论通过查找基因文库中的β-G基因序列并自行设计软件合成引物,检测肝脏及肾脏组织中β-G mRNA基因表达的方法是可行的,对进一步对不同组织中β-G mRNA变化的探讨以及分子机理研究可能有重要意义。

Objective To establish a new method for detection of β-glucuronidase （β-G） mRNA in human liver and kidney tissues. Methods β-G mRNA expression was detected by reverse transcription polymerase chain reaction（RT-PCR） in 10 cases of normal liver tissues, 10 cases of normal kidney tissues and 8 cases of hepatocellular carcinoma tissues. Results The expand products of β-G mRNA were expressed in liver and kidney tissues with similar size of 422 bp. The expression contents of β-G mRNA in liver and kidney normal tissues were different （1.71 ± 0.32 vs 1. 83 ± 0. 22） but without statistical significance （P〉 0. 05）. However, the expression content of β-G mRNA in hepatocellular carcinoma tissues was 3.88±0.86, which was significantly higher （P〈0.01） than that in normal liver tissues. Conclusion β-G mRNA determination is feasible by checking β-G gene alignment in gene bank and designing draw matter in the tissue of liver and kidney. It may be very significant to explore the change of β-G mRNA in various tissues in studying of molecular mechanism.