口服ALA在正常小鼠肺组织中的代谢

The Metabolism of ALA Taking orally on Lung

目的:探求ALA口服在正常小鼠肺组织中的代谢,寻求肺组织光敏诊断最佳的ALA口服剂量及时间。方法:口服不同剂量的ALA(20mg/kg、40mg/kg、60mg/kg、80mg/kg),不同时间(1小时、2小时、4小时、6小时、8小时、10小时、12小时、24小时、48小时)以激光诱导荧光OMA检测系统测定ALA在正常肺组织内的代谢过程。结果:荧光强度在口服ALA40mg/kg组后一小时最强。结论:口服ALA40mg/kg为最适诊断剂量,口服ALA40mg/kg后一小时为最佳检测时间。

Objective： To research on the metabolism of ALA taking orally in lung of normal mice and seek suitable dose and diagnostic time of ALA taking orally. Methods ： Different dose of ALA taking orally（ 20mg/kg, 40mg/kg, 60mg/kg. 80mg/kg）, different time after ALA taking orally（ lh, 2h. 4h. 6h. 8h, 10h. 12h, 24h, 48h）, the fluorescence of lung tissue and tissue was measured when irradiated by laser. By analyzing the spectrum of fluorescence with optical multi-channel analyzer （OMA） the suitable dose and diagnostic time for tumor detection were obtained. Result：The ratio of Ilung tissue/Itissue is largest for 40mg/kg and lh after ALA taking orally. Conclusion：Measuring fluorescence spectrum of lung tissue and tissue and analyzing the spectrum of fluorescence with optical multi-channel analyzer （OMA）, the most suitable dose was 40mg/kg of ALA taking orally, and the measure time is taken 1 hour after ALA taking orally.