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抗肝炎转基因枸杞新品种培育的初步研究 被引量:2

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摘要 以质粒pMVL-HisE制备金粉子弹,通过基因枪轰击枸杞叶片,经壮观霉素筛选获得了抗性完整的再生植株;为探索提高转基因植株同质化程度的可能途径,对再生植株在高浓度的壮观霉素选择压下进行了2轮次的再生抗性筛选,并通过腋芽繁殖的方式继代4-6次,并进一步对枸杞叶绿体转化植株进行PCR扩增分子检测,结果初步证明外源抗肝炎病毒基因已经导入转基因枸杞植株体内。
出处 《宁夏农林科技》 2007年第4期3-4,共2页 Journal of Ningxia Agriculture and Forestry Science and Technology
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