胃癌组织中Sonic Hedgehog和VEGF表达及临床意义的研究

Expressions of Sonic Hedgehog and VEGF in human gastric carcinoma and their clinical significance

目的:探讨胃腺癌中Sonic Hedge-hog(Shh)和VEGF的表达及临床意义。方法:应用免疫组化法检测45例胃癌组织及15例癌旁胃黏膜组织中Shh和VEGF的表达。结果:Shh在胃癌组织中阳性表达率为66.7%,在中、低分化胃癌中的表达高于高分化胃癌中的表达,与组织分化程度相关,P<0.01,在癌旁胃黏膜组织中Shh表达为阴性或弱阳性(15.3%);VEGF在胃癌组织中的阳性表达率(71.1%)显著高于癌旁胃黏膜组织中的阳性表达率(26.7%),P<0.01,与肿瘤分化程度、淋巴结转移呈正相关,P<0.05。Shh和VEGF在胃癌组织中的表达存在相关性(0.01<P<0.05)。结论:Shh在胃癌组织中的表达有一定的特异性,可能是胃癌治疗的一个理想靶目标;Shh与VEGF表达存在相关性,联合检测两者有助于提高胃癌侵袭转移能力的评估,对胃癌的早期诊断、预后判断具有重要的临床意义。

OBJECTIVE： To study the expressions of Sonic Hedgehog （Shh） and vascular endothelial growth factor （VEGF） in gastric carcinoma and their clinical significance. METHODS：The expression of Shh and VEGF in 45 cases of gastric carcinoma tissues and 15 cases of non-cancerous stomach tissues were detected by immunohistochemistry. RESULTS： The positive rates of Shh （66.7%） and VEGF （71. 1%） in gastric cancer were significantly higher than that in non-cancerous stomach tissues （P〈0.01）. The expression of Shh was higher in middle-and-poorly differentiation gastric carcinoma than that in well differentiation gastric carcinoma. The overexpression of Shh in gastric cancer was related to the differentiation of gastric cancer （P〈0.01） ,and that of VEGF was related to lymph node metastasis and the differentiation of gastric cancer（P〈0.05）. The positive correlation was found between the expressions of Shh and VEGF（0.01〈P〈0.05）. CONCLUSIONS： Shh plays an important role in the pathogenesis of gastric cancer. The combined detection of Sonic Hedgehog and VEGF may be of important clinical value for early diagnosis, evaluating the infiltrative ability and prognosis of gastric carcinoma.