Ebselen对大强度耐力训练大鼠心肌自由基代谢和超微结构影响的实验研究

Experiment Research on the Cardiac Muscle Free Radical Metabolism and Ultra-structure Effect of High-intensity Endurance Training Rat Taken by Ebselen

目的探讨Ebselen对大强度耐力训练大鼠心肌组织MDA、T-AOC、NO、NOS系统与心肌超微结构的影响。方法SD雄性健康大鼠30只,适应性饲养、筛选,淘汰个别不适应跑台训练者。将剩余大鼠随机分为3组:安静对照组8只、运动对照组8只、运动加药组8只;进行为期8周的大强度耐力跑台训练。测定心肌组织MDA、T-AOC、NO、NOS含量,透射电子显微镜观察心肌超微结构。结果与安静对照组相比,运动对照组以上各指标均有显著性变化,心肌结构损伤明显;加入药物干预后,各项指标较运动对照组有显著变化,心肌结构明显好转。结论Ebselen对大强度耐力运动导致的自由基损伤有明显的抑制作用,原因可能与Ebselen具有模拟谷胱甘肽过氧化物酶活性有关。

Objective： Discuss an impact of Ebselen on high-intensity endurance training rat cardiac musele organization MDA , T- AOC , NO , NOS system ,and cardiac muscle ultrastructure. Methods： 30 male heahhy SD rats, adaptability raise, sift, eliminate individual one not adapt to minting training. Dividing the random surplus rat into 3 groups： Quiet collation group 8, motion collation group 8, motion adds medicine group 8, and then giving the high-intensity endurance running training which lasting for 8 weeks. Determining enntents of cardiac muscle organization MDA, T-AOC, NO, NOS, and observing the cardiac muscle ultrastructure with transmission electron microscope,. Results： Compared with quiet collation group, all above every index of motion collation group has notable changes, cardiac muscle structure loss is obvious; After being interfered with medicine, various index is notable changes compared with motion collation group and cardiac musele structure also has notable improvement. Conclusion： Ebselen is able to simulate the Glutathione hyperoxide enzymatic activity and to inhibit free radical loss leaded from high-intensity endurance motion obviously, perhaps because it have the enzymatic activity simulating Glutathione hyperoxide.