益肾通肺汤对实验性慢性阻塞性肺疾病大鼠肺组织MMP-9和TIMP-1表达的影响

Effect of Yishentongfei Decoction on MMP-9 and TIMP-1 in Lung Tissue of Rat Model of Chronic Obstructive Pulmonary Disease

目的观察益肾通肺汤对实验性慢性阻塞性肺疾病(COPD)大鼠肺组织中基质金属蛋白酶-9(MMP-9)及其组织抑制因子-1(TIMP-1)表达的影响,以探讨其防治COPD的机理。方法雄性Wistar大鼠36只,随机分为3组:空白组、模型组和治疗组。模型组大鼠两次气管内滴入脂多糖加被动吸烟复制COPD模型。治疗组给予益肾通肺汤。30d后行支气管肺泡灌洗,计数灌洗液中细胞计数及分类,免疫组化方法观察肺组织MMP-9和TIMP-1的表达情况。肺组织切片行苏木素-伊红(HE)染色,观察形态学改变。结果模型组MMP-9和TIMP-1的表达与空白组相比明显增强(P<0.01)。治疗组与模型组相比,MMP-9和TIMP-1的表达相对减弱(P<0.01)。结论益肾通肺汤能降低COPD大鼠肺组织中MMP-9和TIMP-1的表达,起到防治COPD的作用。

Objective To explore the role of prevention and treatment of Yishentongfei decoction in COPD by measuring the expression of MMP-9 and TIMP-1 in lung tissue. Methods 36 male Wistar rats of rat model with chronic obstructive pulmonary disease was established by exposure to cigarette smoke daily for 30 days and injection of LPS in trachea twice. The rats were randomly separated into three groups, the normal group, the model group and the treatment group. Total cell counts and neutrophil counts in BALF were examined. The expression of MMP-9 and TIMP-1 in lung tissue was measured by means of immunohistochemistry （SP）. Lung tissue section stained by HE was observed to study the morphological alternations. Result The expression of MMP-9 and TIMP-1 in lung tissue of the model rats significantly increased compared with the normal rats （P〈0.01）, and decreased relatively compared with the treatment rats （P〈0.01）. Conclusion Yishentongfei decoction can reduce the expression of MMP-9 and TIMP-1 in lung tissue of rat model of COPD, and has effect on the prevention and treatment of COPD.