竹节参总皂苷对脑缺血再灌注模型海马组织一氧化氮合酶的影响

Effects of Total Rhizoma Panacis Japonica Saponins on Nitric Oxide Synthase of Hippocampus Region in the Mouse Repetitious Cerebral Ischemia Reperfusion and the Rat Focal Cerebral Ischemia

目的观察竹节参总皂苷(total rhizoma panacis japonica saponins,tRPJS)对反复脑缺血再灌注小鼠及局灶性脑缺血再灌注大鼠海马组织一氧化氮合酶(NOS)、诱导型一氧化氮合酶(iNOS)的影响,探讨药物抗脑缺血损伤的作用机制。方法插线法阻塞大脑中动脉(MCAO)制备大鼠局灶性脑缺血再灌注模型,采用反复夹闭双侧颈总动脉的方法造成小鼠脑缺血再灌注模型,研究tRPJS对这两种脑缺血再灌注损伤模型海马组织NOS、iNOS的影响。结果tRPJS能显著降低脑缺血再灌注损伤小鼠和局灶性脑缺血大鼠海马组织NOS、iNOS的含量(P<0.05)。结论tRPJS抗脑缺血损伤与降低海马组织NOS、iNOS的活性有关。

Objective To study the mechanism of protective effects of total rhizoma panacis japonica saponins （tRPJS） on the cerebral ischemia injury. Methods The middle cerebral artery occlusion model （MCAO） in rats and cerebral ischemia-reperfusion models in mice were used to investigate the influence of tRPJS on the nitric oxide synthase （NOS） and inducible nitric oxide synthase （iNOS） activity in hippocampus region. Results tRPJS significantly decreased the contents of NOS and iNOS in hippocampus region of MCAO rat and cerebral ischemia reinfusion mouse. Conclusion tRPJS has significantly protective effects by decreasing NOS and iNOS.